A chemiluminescence aptasensor based on Cu/Co nanorods for microcystin-RR detection.

Abstract In this work, a chemiluminescence (CL) aptasensor was designed for detection of microcystin-RR (MC-RR) by introducing Cu/Co nanorods acting as CL catalyzer. Cu/Co nanorods were firstly synthesized throughout a hydrothermal method. Then the thiolated MC-RR aptamer was immobilized on the surface of Cu/Co nanorods, which was employed for recognizing of MC-RR target. In the presence of MC-RR, the conformational change of MC-RR aptamer was happened via recognizing between MC-RR and its aptamer. The luminol CL was catalyzed by Cu/Co nanorods that influenced by conformational change of MC-RR aptamer. The formed MC-RR/aptamer composite prevented the diffusion channel of luminol substrate toward the Cu/Co nanorods surface and restrained the Cu/Co nanorods catalyzing luminol CL. And the intensity of CL depended on the concentration of MC-RR. Under optimal condition, the CL aptasensor had a linear range in 0.1 to 70 nM and a low detection limit of 3.3 × 10−11 M for MC-RR. This study provided a significant method for MC-RR detection in real samples with excellent selectivity. Highlights • Cu/Co BNMs had superior over Cu, Co monometallic nanomaterials and Co(NO 3) 2 , Cu(NO 3) 2. • Cu/Co nanorods were used as nanocatalysts and the carrier for CL detection. • LOD for Microcystin-RR 33 fM was gained. [ABSTRACT FROM AUTHOR]