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Inhibition of Stat3 signaling pathway decreases TNF-α-induced autophagy in cementoblasts.
- Source :
-
Cell & Tissue Research . Dec2018, Vol. 374 Issue 3, p567-575. 9p. - Publication Year :
- 2018
-
Abstract
- Autophagy is a self-digestive process that eliminates impaired or aged proteins and potentially toxic intracellular components to maintain homeostasis. We previously demonstrated that TNF-α played a critical role in cementoblast differentiation, mineralization and apoptosis; however, the effect of TNF-α on cementoblast autophagy has remained unclear. In this study, an elevated immunofluorescence signal of LC3B and autophagic vacuoles, autophagosomes and autolysosomes were detected under TNF-α stimulation in OCCM-30 cells. Autophagy-related genes and proteins, Beclin-1, LC3A and Atg-5, were significantly upregulated by TNF-α in a time- and concentration-dependent manner. During this process, the activity of Stat3 was dramatically enhanced and when the activity of Stat3 was blocked by either a specific chemical inhibitor or siRNA transfection before TNF-α stimulation, the TNF-α-induced upregulation of autophagy-related genes and proteins was strongly inhibited. Our results suggest that TNF-α induced autophagy in cementoblasts was dependent, or partially dependent on the activity of Stat3 signaling pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- *STAT proteins
*TUMOR necrosis factors
*AUTOPHAGY
*HOMEOSTASIS
*APOPTOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 0302766X
- Volume :
- 374
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Cell & Tissue Research
- Publication Type :
- Academic Journal
- Accession number :
- 134039223
- Full Text :
- https://doi.org/10.1007/s00441-018-2890-2