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Label free detection of plant viruses with organic transistor biosensors.
- Source :
-
Sensors & Actuators B: Chemical . Feb2019, Vol. 281, p150-156. 7p. - Publication Year :
- 2019
-
Abstract
- Highlights • Plum Pox Virus affects stone fruit trees and causes severe economic damages. • The PPV biosensor is based on a Electrolyte-Gated Organic Field-Effect Transistor. • Sensitivity and dynamic range are comparable to those of commercially available platforms. Abstract Plum Pox Virus (PPV) is the pathogen responsible for Sharka, a highly infectious disease affecting stone fruit trees and causing severe economic damages, which can be only contained through early-detection and frequent monitoring. We propose a bioelectronic PPV biosensor, based on a electrolyte-gated organic field-effect transistor (EGOFET), for the specific detection of PPV in plant extracts with a sub ng/ml detection limit. The sensing unit of the biosensor is based on anti-PPV antibodies, uniformly oriented on the gold gate electrode by using a sub-monolayer of Protein G. The sensitivity and dynamic range of the EGOFET-based biosensor are comparable to those of commercially available platforms for detection of plant pathogens. This novel electronic immunosensor is compatible with low-cost fabrication procedures and can be easily reconfigured into a fully portable device to be operated in greenhouse and in the field orchards. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PLANT viruses
*TRANSISTORS
*BIOSENSORS
*FIELD-effect transistors
*METAL detectors
Subjects
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 281
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 133972250
- Full Text :
- https://doi.org/10.1016/j.snb.2018.10.080