粉防己碱对神经母细胞瘤干细胞增殖和干性基因表达的影响及作用机制.

BACKGROUND: Studies have shown that tetrandrine can inhibit the characteristics of glioma stem cells by down-regulating the expression of β-catenin. However, its effect on neuroblastoma stem cells has not been reported. OBJECTIVE: To explore the function and mechanism of tetrandrine on proliferation and stemness marker expression of neuroblastoma stem cells. METHODS: Serum-free suspension method was used to obtain SK-N-SH stem cells. SK-N-SH stem cells were treated with tetrandrine, and the inhibitory effect of tetrandrine on the proliferation of neuroblastoma stem cells was detected by MTT method. Immunofluorescence staining was used to detect Nestin expression in the cells after intervention with tetrandrine. The tumorsphere-forming ability of SK-N-SH stem cells was determined by tumorsphere-forming assay. The mRNA levels of CD133, Oct-4 and Nestin were tested by qRT-PCR. The protein level of CD133, Oct-4, Nestin, p-GSK3β and β-catenin were tested by western blot. RESULTS AND CONCLUSION: Tetrandrine inhibited the proliferation of SK-N-SH stem cells (P < 0.05). After treated with tetrandrine, the mRNA levels of CD133, Oct-4 and Nestin in SK-N-SH stem cells were significantly decreased (P < 0.05). Fluorescence intensity of Nestin protien was weakened and the tumorsphere-forming ability of SK-N-SH stem cells was abated (P < 0.05). Tetrandrine also inhibited the protein levels of CD133, Oct-4, Nestin, p-GSK3β and β-catenin (P < 0.05). In conclusion, tetrandrine inhibits the proliferation and stemness marker expression of neuroblastoma stem cells, and the mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway. [ABSTRACT FROM AUTHOR]