THE CHANGES OF STRUCTURE-FUNCTIONAL STATE OF PLASMA MEMBRANE IN NITELLOPSIS OBTUSA UNDER THE INFLUENCE OF DIMETHYLSULFOXIDE.

The change regularity of potential (φm), resistance (Rm) and capacity (Cm) of the plasma membrane (PM) in Nitellopsis obtusa cells under the influence of dimethylsulfoxide (DMSO) have been studied. The investigated cells have φm=-171±3 mV, Rm=3,8±1,6 Om∙m2, Cm=0,92±0,04 mkF∙sm-2 in the standard environmental conditions. The threshold concentration of DMSO 1 ÷ 4 % caused the depolarization of the PM to 20-25mV and increase Cm to 22-26% in the Rm constant. The effective influence of DMSO concentration 1÷ 4% depolarizated PM to the level of -110mV. At first Rm decreased 40%, then occurres its restoration. The final stationary level of Rm was 20% higher than initial one. The value of PM depolarization depended on initial level of φm and the concentration of DMSO in the medium. In that case Cm only increased and its total value was 45% compared with the initial value. In the that work the effects of the solvent are discussed from the point of its influence on the structure state of lipid phase and the transport communications of PM. [ABSTRACT FROM AUTHOR]