Cleavage of arrestin-3 by caspases attenuates cell death by precluding arrestin-dependent JNK activation.

Abstract The two non-visual subtypes, arrestin-2 and arrestin-3, are ubiquitously expressed and bind hundreds of G protein-coupled receptors. In addition, these arrestins also interact with dozens of non-receptor signaling proteins, including c-Src, ERK and JNK, that regulate cell death and survival. Arrestin-3 facilitates the activation of JNK family kinases, which are important players in the regulation of apoptosis. Here we show that arrestin-3 is specifically cleaved at Asp366, Asp405 and Asp406 by caspases during the apoptotic cell death. This results in the generation of one main cleavage product, arrestin-3-(1–366). The formation of this fragment occurs in a dose-dependent manner with the increase of fraction of apoptotic cells upon etoposide treatment. In contrast to a caspase-resistant mutant (D366/405/406E) the arrestin-3-(1–366) fragment reduces the apoptosis of etoposide-treated cells. We found that caspase cleavage did not affect the binding of the arrestin-3 to JNK3, but prevented facilitation of its activation, in contrast to the caspase-resistant mutant, which facilitated JNK activation similar to WT arrestin-3, likely due to decreased binding of the upstream kinases ASK1 and MKK4/7. The data suggest that caspase-generated arrestin-3-(1–366) prevents the signaling in the ASK1-MKK4/7-JNK1/2/3 cascade and protects cells, thereby suppressing apoptosis. Highlights • Arrestin-3 is cleaved by caspases in apoptotic cells. • The main product of caspase cleavage of arrestin-3, (1–366) fragment, localizes to the nucleus. • Arrestin-3-(1–366) has lost the ability to facilitate JNK activation. • Arrestin-3-(1–366) suppresses cell death, whereas caspase-resistant arrestin-3 mutant enhances it. • Arrestin-3-(1–366) serves as a negative feedback in apoptosis. [ABSTRACT FROM AUTHOR]