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Antioxidants Facilitate High-intensity Exercise IL-15 Expression in Skeletal Muscle.
Antioxidants Facilitate High-intensity Exercise IL-15 Expression in Skeletal Muscle.
- Source :
-
International Journal of Sports Medicine . 2019, Vol. 40 Issue 1, p16-22. 7p. - Publication Year :
- 2019
-
Abstract
- Interleukin (IL)-15 stimulates mitochondrial biogenesis, fat oxidation, glucose uptake and myogenesis in skeletal muscle. However, the mechanisms by which exercise triggers IL-15 expression remain to be elucidated in humans. This study aimed at determining whether high-intensity exercise and exercise-induced RONS stimulate IL-15/IL-15Rα expression and its signaling pathway (STAT3) in human skeletal muscle. Nine volunteers performed a 30-s Wingate test in normoxia and hypoxia (PIO2 = 75 mmHg), 2 h after placebo or antioxidant administration (α-lipoic acid, vitamin C and E) in a randomized double-blind design. Blood samples and muscle biopsies (vastus lateralis) were obtained before, immediately after, and 30 and 120 min post-exercise. Sprint exercise upregulated skeletal muscle IL-15 protein expression (ANOVA, P = 0.05), an effect accentuated by antioxidant administration in hypoxia (ANOVA, P = 0.022). In antioxidant conditions, the increased IL-15 expression at 120 min post-exercise (33 %; P = 0.017) was associated with the oxygen deficit caused by the sprint (r = -0.54; P = 0.020); while, IL-15 and Tyr705-STAT3 AUCs were also related (r = 0.50; P = 0.036). Antioxidant administration promotes IL-15 protein expression in human skeletal muscle after sprint exercise, particularly in severe acute hypoxia. Therefore, during intense muscle contraction, a reduced PO2 and glycolytic rate, and possibly, an attenuated RONS generation may facilitate IL-15 production, accompanied by STAT3 activation, in a process that does not require AMPK phosphorylation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *QUADRICEPS muscle physiology
*SKELETAL muscle physiology
*ANTIOXIDANT analysis
*REACTIVE oxygen species
*ANALYSIS of variance
*HYPOXEMIA
*BIOPSY
*BLOOD testing
*CELLULAR signal transduction
*GENE expression
*IMMUNOGLOBULINS
*INTERLEUKINS
*SPRINTING
*MUSCLE contraction
*PHOSPHORYLATION
*PROTEIN kinases
*VITAMIN C
*VITAMIN E
*LIPOIC acid
*RANDOMIZED controlled trials
*ERGOMETRY
*BLIND experiment
*SEVERITY of illness index
*ACUTE diseases
*EXERCISE intensity
*REACTIVE nitrogen species
Subjects
Details
- Language :
- English
- ISSN :
- 01724622
- Volume :
- 40
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- International Journal of Sports Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 133682606
- Full Text :
- https://doi.org/10.1055/a-0781-2527