Effects of some physical agents on Staphylococcus epidermidis and Leishmania tropica: An In vitro study.

This work evaluated the effect of Alpha, Gamma irradiation and Nd:YAG, He- Ne laser on Staphylococcus epidermidis and Leishmania tropica in vitro. The experiment included five replicate of S. epidermidis , L. tropica in vitro exposed to effect of Alpha , Gamma irradiation by 241Am isotopes , in two doses γ = 0.31993 MsV (Milli Sievert) for 3 hr, and in dose α= 1.4157 MsV for 3hr , Americium isotopes give two type of decay Alpha and Gamma Rays. Effect of Nd:YAG laser in 1000 pulse between each pulse 6 sec, in wavelength 1.06 nm ; also He-Ne laser in 5, 10 , 20 , 30 minute , with wavelength 632.8nm. The effect of Nd:YAG, He-Ne laser and Alpha, Gamma irradiation on the viability of S. epidermidis , L. tropica in vitro was determined by using the specific equation compared with Control and determined by using colorimetric MTT assay respectively , the number of viable cells of S. epidermidis , L. tropica was fewer than control (without exposure to laser and irradiation ). Nd:YAG laser, He-Ne laser and Alpha , Gamma irradiation was efficient to kill S. epidermidis and L. tropica [ABSTRACT FROM AUTHOR]