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Direct visualization of single-molecule membrane protein interactions in living cells.
- Source :
-
PLoS Biology . 12/13/2018, Vol. 16 Issue 12, p1-23. 23p. 1 Diagram, 3 Graphs. - Publication Year :
- 2018
-
Abstract
- Interactions between membrane proteins are poorly understood despite their importance in cell signaling and drug development. Here, we present a co-immunoimmobilization assay (Co-II) enabling the direct observation of membrane protein interactions in single living cells that overcomes the limitations of currently prevalent proximity-based indirect methods. Using Co-II, we investigated the transient homodimerizations of epidermal growth factor receptor (EGFR) and beta-2 adrenergic receptor (β2-AR) in living cells, revealing the differential regulation of these receptors’ dimerizations by molecular conformations and microenvironment in a plasma membrane. Co-II should provide a simple, rapid, and robust platform for visualizing both weak and strong protein interactions in the plasma membrane of living cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15449173
- Volume :
- 16
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- PLoS Biology
- Publication Type :
- Academic Journal
- Accession number :
- 133533668
- Full Text :
- https://doi.org/10.1371/journal.pbio.2006660