Adsorption of Heterologous &gamm;-Globulins by Chopped Guinea-Pig Lung.

The amounts of firmly bound γ-globulin (i.e. resistant to elution by Tyrode's solution) showed marked differences when the adsorption of various heterologous γ-globulins on chopped guinea-pig lung was compared [Sheep>pig >bovine>horse>human>rabbit]. This suggests that non-sensitizing γ-globulins were more readily adsorbed than the sensitizing γ-globulins. Only about 30–40 per cent of 131I-labelled protein could be removed from the firmly bound γ-globulin-tissue complex using 8 M urea, suggesting that covalent bonds play a major role in the fixation of γ-globulins to tissues. [ABSTRACT FROM AUTHOR]