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A novel strategy for isolation and purification of fucoxanthinol and fucoxanthin from the diatom Nitzschia laevis.
- Source :
-
Food Chemistry . Mar2019, Vol. 277, p566-572. 7p. - Publication Year :
- 2019
-
Abstract
- Highlights • Nitzschia laevis was a promising resource to develop fucoxanthinol and fucoxanthin production. • A simple two-step method was mediated with SPE and TLC techniques. • This exploited approach could effectively purified fucoxanthinol and fucoxanthin. • The purities of the two purified compounds were over 95%. Abstract In this study, the microalga Nitzschia laevis (N. laevis) can accumulate a marine carotenoid fucoxanthinol. In particular, fucoxanthinol was firstly isolated from microalgae, accompanied by its derivative fucoxanthin. The identification and quantification of fucoxanthinol and fucoxanthin were determined by ultra-performance liquid chromatography coupled to photodiode array detector-quadrupole/travelling-wave ion mobility mass spectrometry/time-of-flight mass spectrometry (UPLC-PDA-TWIMS-QTOF-MS). Furthermore, a cost-effective approach mediated with solid-phase extraction (SPE) and thin-layer chromatography (TLC) technique was used to isolate and purify fucoxanthinol and fucoxanthin from the extracts of N. laevis. This two-step method can obtain 98% fucoxanthinol and 95% fucoxanthin, with the recovery efficiencies of around 85% for fucoxanthinol and 70% for fucoxanthin, respectively. Moreover, 1H and 13C nuclear magnetic resonance (NMR) techniques were adopted to record the purified compounds for supporting the above results. In all, the developed method has a promising potential to purify fucoxanthinol and fucoxanthin of microalgae for food and pharmaceutical applications. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03088146
- Volume :
- 277
- Database :
- Academic Search Index
- Journal :
- Food Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 133257887
- Full Text :
- https://doi.org/10.1016/j.foodchem.2018.10.133