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SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice.
- Source :
-
Developmental Cell . Dec2018, Vol. 47 Issue 5, p645-645. 1p. - Publication Year :
- 2018
-
Abstract
- Summary Meiotic synapsis and recombination ensure correct homologous segregation and genetic diversity. Asynapsed homologs are transcriptionally inactivated by meiotic silencing, which serves a surveillance function and in males drives meiotic sex chromosome inactivation. Silencing depends on the DNA damage response (DDR) network, but how DDR proteins engage repressive chromatin marks is unknown. We identify the histone H3-lysine-9 methyltransferase SETDB1 as the bridge linking the DDR to silencing in male mice. At the onset of silencing, X chromosome H3K9 trimethylation (H3K9me3) enrichment is downstream of DDR factors. Without Setdb1 , the X chromosome accrues DDR proteins but not H3K9me3. Consequently, sex chromosome remodeling and silencing fail, causing germ cell apoptosis. Our data implicate TRIM28 in linking the DDR to SETDB1 and uncover additional factors with putative meiotic XY-silencing functions. Furthermore, we show that SETDB1 imposes timely expression of meiotic and post-meiotic genes. Setdb1 thus unites the DDR network, asynapsis, and meiotic chromosome silencing. Graphical Abstract Highlights • The histone methyltransferase SETDB1 is essential for male mouse meiosis • The meiotic DDR network recruits SETDB1 to the XY pair, where it induces H3K9me3 • SETDB1 deletion perturbs meiotic sex chromosome remodeling and silencing • SETDB1 ensures timely expression of meiotic and post-meiotic genes During male meiosis in mammals, the asynapsed regions of the X and Y chromosomes retain DNA double-strand breaks (DSBs), which triggers silencing of the sex chromosomes, a process essential for fertility. Hirota et al. show that meiotic DSB factors recruit the H3K9-methyltransferase SETDB1, inducing XY-chromatin remodeling and silencing. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MEIOSIS
*SEX chromosomes
*DNA damage
*CHROMATIN
*METHYLTRANSFERASES
Subjects
Details
- Language :
- English
- ISSN :
- 15345807
- Volume :
- 47
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Developmental Cell
- Publication Type :
- Academic Journal
- Accession number :
- 133256220
- Full Text :
- https://doi.org/10.1016/j.devcel.2018.10.004