A lateral flow assay for simultaneous detection of Deoxynivalenol, Fumonisin B1 and Aflatoxin B1.

Abstract Deoxynivalenol (DON), Fumonisin B 1 (FB 1) and Aflatoxin B 1 (AFB 1) have strong toxicity to humans and exist widely in grain and food. It is necessary to screen these mycotoxins to guarantee food safety. In order to develop a rapid, simple, low-cost and simultaneous-detection method, composites of antibody-nano-Au-particles, DON-BSA, FB 1 -BSA, and AFB 1 -BSA were prepared to establish a lateral flow assay based on competitive inhibition. The results suggested that the visual detection limits for DON, FB 1 and AFB 1 were 10, 30, and 10 ng mL−1, respectively. On the other hand, it had no reactivity to T-2 toxin, zearalenone, ochratoxin A and nivalenol, which demonstrates good specificity. Besides, the strip had good repeatability and stability. Therefore, DON, FB 1 and AFB 1 would be screened simultaneously by lateral flow assay for food safety. Highlights • A lateral flow assay for simultaneous detection of DON, FB 1 and AFB 1. • The visual detection limit was down to 10 ng mL−1. • The assay showed high stability and specificity. [ABSTRACT FROM AUTHOR]