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Enhanced microbial lipid production by Cryptococcus albidus in the high-cell-density continuous cultivation with membrane cell recycling and two-stage nutrient limitation.
- Source :
-
Journal of Industrial Microbiology & Biotechnology . Dec2018, Vol. 45 Issue 12, p1045-1051. 7p. 1 Diagram, 4 Charts, 2 Graphs. - Publication Year :
- 2018
-
Abstract
- As a potential feedstock for biofuel production, a high-cell-density continuous culture for the lipid production by Cryptococcus albidus was investigated in this study. The influences of dilution rates in the single-stage continuous cultures were explored first. To reach a high-cell-density culture, a single-stage continuous culture coupled with a membrane cell recycling system was carried out at a constant dilution rate of 0.36/h with varied bleeding ratios. The maximum lipid productivity of 0.69 g/L/h was achieved with the highest bleeding ratio of 0.4. To reach a better lipid yield and content, a two-stage continuous cultivation was performed by adjusting the C/N ratio in two different stages. Finally, a lipid yield of 0.32 g/g and lipid content of 56.4% were obtained. This two-stage continuous cultivation, which provided a higher lipid production performance, shows a great potential for an industrial-scale biotechnological production of microbial lipids and biofuel production. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CRYPTOCOCCUS
*LIPIDS
*BIOMASS energy
*CELL determination
*DILUTION
Subjects
Details
- Language :
- English
- ISSN :
- 13675435
- Volume :
- 45
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Journal of Industrial Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 133160453
- Full Text :
- https://doi.org/10.1007/s10295-018-2081-x