Effect of external resistance on the sensitivity of microbial fuel cell biosensor for detection of different types of pollutants.

Abstract The relatively poor sensitivity is the main bottleneck restricting the application of microbial fuel cell biosensor (MFC-biosensor) for toxicity monitoring. Previous studies have shown that external resistance (R ext) had an obvious effect on sensor sensitivity. However, these studies reported different results and the reason of this discrepancy was not clear. The objective of this research was to observe the effect of R ext on sensor sensitivity when detecting different types of pollutants and reveal its microbiological mechanism. Results demonstrated that the optimal R ext of MFC-biosensor varied with the type of pollutants. The optimal values for detecting avermectins, tetracyclines and heavy metals were 100 Ω, 330 Ω and 680 Ω, respectively. This discrepancy was mainly due to the visible differences in anodic microbial communities at different R ext settings. Both Azospirillum and Acinetobacter were susceptible to Cd and Pb, occuping 19.20% of the anodic microbial population in 680 Ω MFC-biosensor. Pseudomonas accounted for 10.73% in 330 Ω MFC-biosensor and possessed the sensitivity to tetracyclines. As for 100 Ω MFC-biosensor, the avermectin-intolerant Ocillibacter made up 2.55% of the anodic microbial community. This study indicated that the R ext of MFC-biosensor should be optimized according to the potential pollutants. Graphical abstract Unlabelled Image Highlights • Optimal external resistance of MFC-biosensor varies with the type of pollutants. • The optimal external resistance for detecting heavy metals is 680 Ω. • The optimal value for avermectins is 100 Ω while 330 Ω for tetracyclines. • The discrepancy is attributed to the differences in anodic microbial communities. [ABSTRACT FROM AUTHOR]