Skin secretion transcriptome remains in chromatographic fractions suitable for molecular cloning.

Abstract Traditional sources of mRNA for molecular cloning on amphibian skin secretion studies have been the frog's skin and skin secretions. Here, we demonstrate that mRNA isolated from chromatographic fractions of skin secretions is amenable for molecular cloning assays. We identified precursor sequences of the Arg0, Trp5, Leu8-bradykinin and six antimicrobial peptides of Pelophylax esculentus (Ranidae). These results show that both transcriptomic and peptidomic analyses can be performed with a single sample reducing in half the amount of starting skin secretion required. This is a significant advantage when working with endangered or very rare amphibian species, where minimal samples are available. Highlights • mRNA isolated from chromatographic fractions of skin secretions is amenable for molecular cloning assays. • Seven antimicrobial peptides precursor sequences of Pelophylax esculentus were identified by cloning of FPLC fractions. • mRNA and peptides co-elution suggest an interaction between these molecules in chromatographic fractions. [ABSTRACT FROM AUTHOR]