THIN-LAYER CHROMATOGRAPHY ANALYSIS OF EXTRACT OXYTROPIS GLABRA LAM. DC.

Background: In recent years, researchers are increasingly interested in plant raw materials containing phenolic compounds, including flavonoids, due to their harmlessness and a wide range of biological activity. In plants and biological fluids flavonoids are found mainly in the form of a variety of glycosides. One of the most promising plant sources of phenolic compounds is Oxytropis glabra Lam.DC. In this regard, the issue of rapid and qualitative assessment of biologically active substances in medicinal plant raw materials becomes relevant. In this case, application of thin-layer chromatography method is the most optimal solution Thin-layer chromatography is one of the simplest and most well-known variants of liquid chromatography, in which the separation of the mobile phase components occurs on the open layer of the sorbent. The advantages of this method include speed and ease of implementation, as well as low costs. However, the development and selection of optimal methods for research can represent significant complexity. The aim of this work was identification of methanol extracts by the thin-layer chromatography method. Using a Soxhlet extractor has been obtained four methanol extracts. The purpose of our research was a comparative study of the vegetative organs (Flowers, stem, leaves, root) of Oxytropis glabra Lam.DC. Methods: Populations of Oxytropis glabra Lam.DC., family Fabaceae were collected in Akkol forestry, which is located within the Balkhash region and the Ili river. The climate of Southern Balkhash is sharply continental. The coldest month is January (the average air temperature is 25°C), the hottest is July (+25 ° C). Winter lasts for 4-4.5 months. Spring begins in March-April and lasts for about 2 months, being characterized by a rapid increase in heat, an increase in precipitation and wind speed. Summer is hot, arid, comes in the second half of May and lasts for 120-130 days. Sometimes dry winds are observed, the temperature reaches +35, +40°C, and the soil is heated to +50, +60°C. The annual amount of precipitation does not exceed 135-150 mm. The wettest months are April, May, and the least humid months are August-September. The prevailing winds are of the northeastern and eastern directions; hydromorphic soils, solonchaks, takyrlike and desert sandy soils reach the greatest extent. The qualitative composition of the methanol extracts was determined by thin layer chromatography (TLC) using high-performance plates TLC Silica gel 60 F254, TLC cellulose 10x10 cm, HPLTC silica gel 60 DIOL F254S, 10×10 cm, HPLTC silica gel 60 RP - 18W, 10×20 cm, TLC silica gel 60, 20×20 cm, (Merck, Germany). Plant material was exhaustively extracted at Soxhlet apparatus with chloroform (purification from lipophilic compounds, 6 day (144 hour)) and next with methanol (flavonoids), 5 day (120 hour). The resulting extract was evaporated by a rotary evaporation apparatus, and the extract was made up to a mark with 50 ml of methanol. Results: The results of the study allow us to state that the selected butanol-methanol-water solvent system (300: 50: 50), and using 15% acetic acid promote the optimal separation of extract components. On the plates of the brand "TLC cellulose" and TLC silica gel 60 were observed clearer spots. As a result, were extracted 17 components from flowers, 17 components from leaves, 8 components from the stem. [ABSTRACT FROM AUTHOR]