Effects of Sodium Nitroprussiate on the Metabolites and Key Enzyme Activities of Carbon-nitrogen Metabolism from Seed Germination to Seedling Period of Cherry Tomato.

This study was conducted to elucidate the regulating mechanism of sodium nitroprussiate on seed germination and seedling growth of cherry tomato. After the treatment with different concentrations of sodium nitroprusside, the effects on the metabolites and key enzyme activities in carbon-nitrogen metabolism of cherry tomato cultivar Zhuyun as an experimental material were investigated. The results showed that from seed germination to seedling period, the contents of starch and total nitrogen decreased, but the contents of soluble sugar, reducing sugar, sucrose and soluble protein increased firstly and then decreased; and free amino acids content increased gradually. Sodium nitroprusside made the contents of starch, sucrose and fiee amino acids higher than CK. From seed germination to seedling period in cherry tomato, the activities of amylase, glutamine synthetase (GS) and sucrose phosphate synthase (SPS) decreased; the activities of nitrate reductase (NR) and glutamate synthase (GOGAT) increased at first and decreased then; and the CK and the 0.25 mmol/L sodium nitroprusside treatments exhibited decreased sucrose synthase (SS) activity, and the trend was increasing at first and decreasing then after the treatment with 0.50 and 1.00 mmol/L sodium nitroprusside. Sodium nitroprusside treatment improved amylase activity; and the carbon-nitrogen ratio of the CK increased at first and decreased then, while the values of other treatments tended to decrease. In addition, sodium nitroprusside did not affect seed germination potential and germination rate, but significantly improved biomass accumulation, root length and height of seedlings. These data suggest that sodium nitroprusside could affect the conversion of starch and sugar accumulation, delay the decomposition of total nitrogen and soluble protein, and achieve the effects of accelerating the accumulation of free amino acids, and promoting seed germination and seedling growth, and 0.50 mmol/L sodium nitroprusside has the best effect. [ABSTRACT FROM AUTHOR]