Back to Search
Start Over
Site-specific incorporation of quadricyclane into a protein and photocleavage of the quadricyclane ligation adduct.
- Source :
-
Bioorganic & Medicinal Chemistry . Oct2018, Vol. 26 Issue 19, p5280-5290. 11p. - Publication Year :
- 2018
-
Abstract
- Graphical abstract Abstract The quadricyclane (QC) ligation is a bioorthogonal reaction between a quadricyclane moiety and a nickel bis(dithiolene) derivative. Here we show that a QC amino acid can be incorporated into a protein site-specifically using the pyrrolysine-based genetic code expansion platform, and subsequently used for ligation chemistry. Additionally, we exploited the photolability of the QC ligation product to render the adduct cleavable with a handheld UV lamp. We further developed a protein purification method that involves QC ligation of biotin to a protein of interest, capture on streptavidin resin, and finally release using only UV light. The QC ligation thus brings novel chemical manipulations to the realm of bioorthogonal chemistry. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09680896
- Volume :
- 26
- Issue :
- 19
- Database :
- Academic Search Index
- Journal :
- Bioorganic & Medicinal Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 132919561
- Full Text :
- https://doi.org/10.1016/j.bmc.2018.04.009