有氧运动与黑枸黄酮干预酒精性肝损伤模型小鼠炎性因子的变化.

BACKGROUND: There is still a lack of an effective treatment method for alcoholic liver injury. Extracts from Lycium ruthenicum Murr have been shown to play a role in immunoregulation, reducing blood lipid and glucose, antioxidation, prevention and treatment of cardiovascular disease and tumor. OBJECTIVE: To investigate the effect of aerobic exercise combined with flavonoids from Lycium ruthenicum Murr on alcoholic liver injury and its anti-inflammatory mechanism. METHODS: Forty healthy male Kunming mice were used to construct alcoholic liver injury models. The rat models were randomly divided into model, flavonoids, aerobic exercise and aerobic exercise + flavonoids from Lyceum ruthenicum Murr (combination) groups. Aerobic exercise and intragastrical administration of flavonoids from Lyceum ruthenicum Murr were given at 1 day after modeling for 6 weeks. Afterwards, the mice were fasted overnight and then the liver tissues were removed. Hematoxylin-eosin staining and histopathological scoring were conducted. The serum contents of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase and total bilirubin were tested by biochemical analysis, and the mRNA expression levels of tumor necrosis factor α, nuclear factor κB, interleukin 6 and interleukin 10 in the liver tissues and blood were determined by real-time PCR. RESULTS AND CONCLUSION: Hematoxylin-eosin staining and histopathological changes: in the model group, the hepatic cord arranged in disorder, hepatic lobules were damaged severely, and there were abundant uneven fat vacuoles and inflammatory cells in the cytoplasm. The damage degree of liver tissues was significantly lowered in the flavonoids and aerobic exercise groups (P < 0.05). The necrotic hepatocytes, fat vacuoles and inflammatory cell infiltration were significantly decreased in the combination group (P < 0.01). Compared with the model group, the serum contents of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase and total bilirubin in the combination (P < 0.01) and aerobic exercise (P < 0.05) groups were significantly reduced. Real-time PCR results: compared with the model group, the mRNA expression levels of tumor necrosis factor α, nuclear factor κB, interleukin 6 in the liver tissues and blood in the three treatment groups were significantly reduced (P < 0.05), especially in the combination group (P < 0.01), and the mRNA expression level of interleukin 10 was significantly increased. Our findings manifest that aerobic exercise and flavonoids from Lycium ruthenicum Murr can effectively reduce the expression levels of inflammatory cytokines. As a minimally invasive early detection method, circulating RNA detection of plasma inflammatory factors is an important marker in the screen, observation and prognosis of alcoholic liver injury. [ABSTRACT FROM AUTHOR]