Impact of domestication on the evolution of rhizomicrobiome of rice in response to the presence of Magnaporthe oryzae.

Abstract The rhizomicrobiome plays a key role in suppressing soil-borne plant diseases. It remains unclear if crop domestication has altered the rhizomicrobiome and reduced the resistance of domesticated crops to pathogens. To investigate this question, the pathogenic fungus Magnaporthe oryzae was administered to the rhizosphere of plants of cultivated and wild rice to compare the impact of the fungal pathogen on their rhizomicrobiome. The analysis of the results indicated that the presence of M. oryzae affected the community structure and diversity of the rhizomicrobiome of both cultivated and wild rice species. Bacterial and fungal α- and β-diversity of the rhizosphere of cultivated rice were altered more significantly than in wild rice. Furthermore, the abundance of the introduced pathogen was significantly lower in the rhizosphere of wild rice, while the relative abundance of putatively beneficial bacterial and fungal taxa was higher, relative to cultivated rice. These results suggest that the rhizomicrobiome of cultivated rice was more sensitive to the introduction of the fungal pathogen and more easily disturbed than the rhizosphere community of its wild relative. Additionally, a correlation analysis of microbiome and root transcriptome data, obtained under pathogenic and non-pathogenic conditions, indicated that fungal members of the Glomeromycota are important for promoting phenylpropanoid and lignin syntheses in wild rice, which plays a role in resisting M. oryzae infection. The identified differences between the responses of the rhizomicrobiomes of cultivated and wild rice to M. oryzae may provide information that can be used in developing novel strategies to control soil-borne pathogens, which include reconstructing the rhizomicrobiome of domesticated crops to be similar to their wild relatives. Highlights • Rhizomicrobiomes of both cultivated and wild rice are affected by the pathogen Magnaporthe oryzae. • Rhizomicrobiome of cultivated rice is more sensitive to M. oryzae than that of wild rice. • Glomeromycota fungi might play an important role in the resistance of wild rice to M. oryzae. [ABSTRACT FROM AUTHOR]