Benzyladenine-induced stimulation of two components of chlorophyll formation in etiolated cucumber cotyledons.

Excised etiolated cotyledons of cucumber (Cucumis sativus L. cv. Aonagajibai) were continuously irradiated under various intensities of white light. The rate of chlorophyII (Chi) formation during the lag phase reaches a plateau at fluence rates above 1.4 urmol m−2 s−1. This is true in both water‐control and benzyladenine (BA)‐pretreated cotyledons. In cotyledons pretreated for 14 h with BA in darkness (in which case, Chl formation is stimulated by BA during both the lag and the steady‐state phases), the increase in the steady‐state rate of Chl formation with increasing light in tensity is stimulated compared to that of the water control over the range of fluence rates, 0. 25‐43 urmol m−2 s−1. In cotyledons pretreated for 6 h with BA in darkness (only Chl formation during the lag phase is stimulated), only an increase in fluence rate from 0.25 to 1.4 umol m−2 s−1 causes a higher increase in the Chl formation in the BA‐treated cotyledons than in the water control. The time course of Chl formation shows that the BA‐induced late‐appearing effect (stimulation of the steady‐state rate) is almost absent at low intensity illumination, but the BA‐induced fast‐appearing effect (elimination of the lag phase) is effective at all intensities. From this evidence, the Chl‐forming process apparently consists of two components, whose periods of operation or light‐intensity requirements are different. BA stimulates the rates of the respective components in both the fast and the late‐appearing effects. [ABSTRACT FROM AUTHOR]