RNA干扰敲低β-catenin 的表达对瘢痕疙瘩成纤维细胞增殖及凋亡的影响.

Objective: To investigate the effect of β-catenin siRNA on the proliferation and apoptosis of keloid fibroblast cell. Methods: Real-time PCR and Western blot were performed to monitor the mRNA and protein expression of β-catenin in pathological scar tissue and adjacent normal tissue. Keloid fibroblast cell in vitro were transfected with 3 pairs of specific β-catenin small interfering RNA (siRNA), then Real-time PCR and Western blot were performed to identify the best siRNA. The proliferation and apoptosis of KFB transfected with β-catenin siRNA were detected by using MTT assay and flow cytometry. Results: The RT-PCR and Western blot assay results showed that the expression of β-catenin mRNA and protein in pathological scar tissue were significantly higher than those in the adjacent normal tissue (P< 0.05). The (3-catenin siRNA 2 remarkably inhibited the expression of β-catenin at mRNA and proteins in the Fiuman keloid fibroblasts. Compared with the control group, cell proliferation was decreased and apoptotic rate were increased in β-catenin siRNA 2 group. Conclusion: Knockdown of β-catenin significantly decreased the proliferation and increased the apoptosis of KFB, which could inhibit the formation of pathological scar. [ABSTRACT FROM AUTHOR]