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Quantitative Detection of Pork Contamination in Cooked Meat Products by ELISA.
- Source :
-
Journal of AOAC International . May/Jun2018, Vol. 101 Issue 3, p810-816. 7p. 9 Charts, 3 Graphs. - Publication Year :
- 2018
-
Abstract
- Recent news of many cases of adulteration of meats with pork has bolstered the need for a way to detect and quantify the unwanted contamination of pork in other meats. To address this need, Microbiologique, Inc. has produced a sandwich ELISA assay that can rapidly quantify the presence of pork in cooked horse, beef, chicken, goat, and lamb meats. We carried out a validation study and showed that this assay has an analytical sensitivity of 0.00014 and 0.00040% (w/v) for cooked and autoclaved pork, respectively, and an analytical range of quantitation of 0.05-3.2% (w/v) in the absence of other meats. The assay can measure pork contamination down to 0.1% (w/w) in the presence of cooked horse, beef, chicken, goat, and lamb meats. The assay is quick and can be completed in 1 h and 10 min. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10603271
- Volume :
- 101
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of AOAC International
- Publication Type :
- Academic Journal
- Accession number :
- 132274230
- Full Text :
- https://doi.org/10.5740/jaoacint.17-0036