Effects of chlortetracycline on biological nutrient removal from wastewater.

Abstract Due to the widespread use of antibiotics in healthcare and livestock production, antibiotic resistance genes and residual antimicrobials would enter environment and further discharge into the municipal sewage system. The objective of this work was to explore the potential effect of chlortetracycline (CTC) on biological nutrient removal from wastewater. Thus, the effects of CTC on biological phosphorus and nitrogen removal were investigated with respect to the viability of bacteria, the activities of key metabolic enzymes, and the transformations of intermediate metabolites. Results showed that the presence of 0.1 mg·L−1 CTC did not show any impact on biological phosphorus and nitrogen removal. Nevertheless, the long-term exposure to 1 and 10 mg·L−1 CTC decreased TN removal efficiency from 77.4% to 64.1% and 53.4%, respectively. Meanwhile, the presence of 10 mg·L−1 CTC decreased the SOP removal efficiency from 96.3% to 78.1%. Mechanism studies indicated that CTC could affect the activities of reductase and the transformations of polyhydroxyalkanoates and glycogen, resulting in inhibition of denitrification and phosphorus uptake, which may be the major reason for the high level of CTC showing adverse influence on wastewater biological nutrient removal. Graphical abstract This study showed the long-term exposure to mg·L−1 CTC inhibited denitrification and phosphorus uptake by affecting the transformations of PHAs and glycogen and decreasing the activity of NIR. Unlabelled Image Highlights • The high level of CTC could affect nitrogen and phosphorus removal. • Microbial enzymatic activities were analyzed at different CTC concentration. • Nitrogen and phosphorus removal rate was related to microbial enzymatic activity. • The transformations of PHAs and glycogen were suppressed by CTC addition. [ABSTRACT FROM AUTHOR]