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A ratiometric fluorescent probe for hypochlorous acid and its biological applications.

Authors :
Liu, Chang
Wang, Qing
Jiao, Xiaojie
Yao, Huirong
He, Song
Zhao, Liancheng
Zeng, Xianshun
Source :
Dyes & Pigments. Jan2019, Vol. 160, p989-994. 6p.
Publication Year :
2019

Abstract

Abstract We present a lysosome-targetable and ratiometric fluorescent probe, TJ2 , for visually monitoring exogenous and endogenous HOCl in living cells by using a built-in S atom within the π-extended rhodamine fluorophore as a pH-independent recognition site. Based on the oxidation-promoted intramolecular charge transfer processes, TJ2 demonstrates fast ratiometric fluorescence response, excellent selectivity, and high sensitivity (2.3 nM) toward HOCl. Furthermore, TJ2 is highly lysosome-targetable, which could be used for ratiometric monitoring of the levels of HOCl in lysosomes. Graphical abstract A ratiometric lysosome-targetable fluorescent probe has been designed and synthesized for the sensitive and selective detection of HOCl. Image 1 Highlights • A ratiometric probe TJ2 has been synthesized for lysosomal HOCl imaging. • The probe showed fast fluorescence response, excellent selectivity, and high sensitivity (2.3 nM) toward HOCl. • The probe could be used for ratiometric monitoring of the level of lysosomal HOCl in living cells in real time. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01437208
Volume :
160
Database :
Academic Search Index
Journal :
Dyes & Pigments
Publication Type :
Academic Journal
Accession number :
131999014
Full Text :
https://doi.org/10.1016/j.dyepig.2018.07.001