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Biocatalytic Synthesis of γ-glutamyl-L-leucine, a Kokumi-Imparting Dipeptide, by Bacillus licheniformis γ-Glutamyltranspeptidase.
- Source :
-
Food Biotechnology . 2018, Vol. 32 Issue 2, p130-147. 18p. 8 Graphs. - Publication Year :
- 2018
-
Abstract
- Several γ-glutamyl compounds are known to have favorable features for a variety of biotechnological and pharmaceutical applications. However, so far only a limited number of γ-glutamyl compounds have been synthesized through γ-glutamyltranspeptidase (GGT). With the help of thin layer chromatography and computer-assisted image analysis techniques, we performed an enzymatic method involving Bacillus licheniformis GGT (BlGGT) for the synthesis of γ-glutamyl-L-leucine (γ-Glu- Leu). The optimum conditions for a biocatalytic synthesis of γ- Glu-Leu were determined to be 200 mM glutamine, 200 mM leucine, 50 mM Tris-HCl buffer (pH 9.0), and BlGGT at a working concentration of 2.0 U/mL. Upon a 5-h incubation of the reaction mixture at 50°C, the product yield could reach approximately 52%. The preparative synthesis of γ-Glu-Leu by BlGGT was done under optimal conditions at millimol scale level. The reaction products were subjected to mass spectrometry/nuclear magnetic resonance analyses to ensure the successful synthesis of γ-Glu-Leu. Taken together, the present study demonstrates the high potentiality of BlGGT-catalyzed reaction in the synthesis of γ-glutamyl compounds. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08905436
- Volume :
- 32
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Food Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 131980146
- Full Text :
- https://doi.org/10.1080/08905436.2018.1444636