High Performance of Covalently Grafting onto Collagen in The Presence of Graphene Oxide.

A collagen-based copolymer, ASC-g-Poly(methyl methacrylate-co-Ethyl Acrylate), was synthesized in the presence of Graphene Oxide (GO) via an in-situ polymerization. The presence of GO that increased the accessible surface area for initiated collagen chains allowed for an accelerated polymerization with highly improved grafting performance and efficiency. This was conducted from two polymerization systems with varied comonomer feed ratios, in which two distinguished GO loadings were used. The processability of the achieved nanocomposite was then evaluated through casting and electrospinning processing methods. Fourier Transform Infrared Spectroscopy (FT-IR), UV-Vis spectroscopy, Differential Scanning Calorimeter (DSC), Thermogravimetric analysis (TGA), Scanning Electron Microscope (SEM), Transmission electron microscopy (TEM), and tensile analysis were conducted to characterize the GO-ASC-g-P(MMA-co-EA). The nanocomposite films showed a unique morphology, multilayer nanostructure of the grafted GO monolayers that deposited simultaneously one on top of another. The morphology of the electrospun fibers was affected by the addition of GO loadings in which the increase in fiber diameter was observed while the surface of the nanofibers was decorated by the GO nanolayers. To modify the collagen, this research highlights the importance of introducing functional groups of GO and the substitution of GO loadings as an active nanostructure filler to highly monomer feed ratios improving the physiochemical properties of collagen. This easy-to-apply approach is suggested for applications intending the mechanical properties and deterred degradation of Collagen-based materials. [ABSTRACT FROM AUTHOR]