国产多孔钽对兔成骨细胞生物学行为及功能的影响.

BACKGROUND: The domestic porous tantalum implant material has a three-dimensional spatial structure that may affect the growth and secretion of cells. OBJECTIVE: To investigate the proliferation, cell cycle, osteogenic secretion of osteoblasts co-cultured with domestic porous tantalum scaffolds. METHODS: Passage 3 rabbit osteoblasts were extracted and divided into three groups: normal culture group, control group added with the porous tantalum extract, and experimental group co-cultured with the porous tantalum scaffold. The proliferation of cells in each group was detected by cell counting kit-8 method at 3, 5, 7 days of culture. The growth of osteoblasts on the porous tantalum scaffold was observed by scanning electron microscopy. ELISA kit assay was applied to detect the levels of osteocalcin and type I collagen in osteoblasts at 3, 5, 7 days of culture. The cell circle of osteoblasts was detected by flow cytometry at 7 days of culture. RESULTS AND CONCLUSION: (1) Cell proliferation: There was no difference in cell proliferation among the three groups at different time points (P > 0.05). (2) Scanning electron microscopy: On the 3rd day of compound culture, the cells adhered to the scaffold surface and pores, and arranged sparsely with few protrusions. On the 5th day, the cells began to extend, and the adjacent cells were interconnected to form a flaky growth. On the 7th day, the cells merged into pieces, and the secreted matrix covered most of the scaffold surface. (3) ELISA detection: With the cultivation time, the osteocalcin secretion from the osteoblasts was gradually increased in all the three groups, while the secretion of type I collagen was increased first and then decreased. The secretion of osteocalcin and type I collagen in the experimental group at different time points were higher than that in normal culture and control groups. (4) Cell cycle: The osteoblasts in the three groups were normal diploid cells, no aneuploid cells appeared, and the cell DNA content was normal. The cell cycle distribution was similar in the three groups. To conclude, the domestic porous tantalum scaffolds have good biocompatibility and may promote osteoblast mineralization and osteogenesis. [ABSTRACT FROM AUTHOR]