近红外荧光染料IR-780在肾透明细胞癌中的成像特征.

BACKGROUND: The near-infrared fluorescent dyes that directly bind to tumor cells have been developed, and IR-780 stands out. OBJECTIVE: To evaluate the specific imaging effect of near-infrared fluorescent dye IR-780 in clear cell renal cell carcinoma (ccRCC). METHODS: Human ccRCC cells 786-O, ACHN and normal human embryonic kidney 293T cells in logarithmic growth phase were incubated with 20 μmol/L IR-780 for 30 minutes. The localization of the dye in ccRCC was observed by laser confocal microscopy. Human ccRCC cells 786-O in logarithmic phase was incubated with 20 μmol/L IR-780 for 30 minutes. The localization of the dye in mitochondria and lysosomes was observed under confocal laser scanning microscope. 10, 100, 1 000 and 10 000 DAPI-stained human ccRCC cells 786-O were respectively added to human blood samples. The mononuclear cells in each group were isolated and cultured with 20 μmol/L IR-780 for 30 minutes. DAPI and IR-780 double positive cells were observed under confocal laser scanning microscope. RESULTS AND CONCLUSION: IR-780 possessed the ability to visualize a variety of ccRCC cells, but not for normal renal epithelial cells. IR-780 had obvious overlapping staining in lysosomes or mitochondria, indicating its selective accumulation in mitochondria and lysosomes in the organelles. IR-780 could detect a small amount of ccRCC in the blood. These results suggest that the near-infrared fluorescent dye IR-780 can specifically accumulate in ccRCC cells, which can be used for specific diagnosis of ccRCC. [ABSTRACT FROM AUTHOR]