Efficient transformation of Pleurotus eryngii with a safe selective marker mutated from the Pesdi1 gene.

Abstract We introduced a site-directed mutation in the sdi1 gene and used it as a selective marker for the polyethylene glycol-mediated transformation of Pleurotus eryngii monokaryon protoplasts. The transformants displayed obvious and stable resistance to the fungicide carboxin indicating that the mutant Pesdi1 gene is an efficient selective marker. [ABSTRACT FROM AUTHOR]