Comparison of three DNA extraction methods for molecular confirmation of Mycobacterium avium subspecies paratuberculosis from the VersaTrek™ liquid cultures of bovine fecal samples.

Abstract We evaluated three DNA extraction methods for confirmation of Mycobacterium avium subspecies paratuberculosis from liquid cultures of bovine feces. Use of DNA Extract All Reagents Kit™ resulted in efficient extraction of amplifiable DNA from higher proportion (96.29%) of known positive samples compared to Chelex-100 resin (25.92%) and polyethylene glycol (0%). Highlights • Johne's disease is caused by Mycobacterium avium subsp. paratuberculosis (MAP). • PCR is used for confirmation of MAP in liquid cultures for the disease diagnosis. • Use of DNA Extract All Reagents Kit™ resulted in efficient extraction of MAP DNA. [ABSTRACT FROM AUTHOR]