Back to Search
Start Over
Whole‐Cell Biotransformation of Benzene to Phenol Catalysed by Intracellular Cytochrome P450BM3 Activated by External Additives.
- Source :
-
Angewandte Chemie International Edition . 9/17/2018, Vol. 57 Issue 38, p12264-12269. 6p. - Publication Year :
- 2018
-
Abstract
- Abstract: An Escherichia coli whole‐cell biocatalyst for the direct hydroxylation of benzene to phenol has been developed. By adding amino acid derivatives as decoy molecules to the culture medium, wild‐type cytochrome P450BM3 (P450BM3) expressed in E.coli can be activated and non‐native substrates hydroxylated, without supplementing with NADPH. The yield of phenol reached 59 % when N‐heptyl‐ l‐prolyl‐ l‐phenylalanine (C7‐Pro‐Phe) was employed as the decoy molecule. It was shown that decoy molecules, especially those lacking fluorination, reached the cytosol of E. coli, thus imparting in vivo catalytic activity for the oxyfunctionalisation of non‐native substrates to intracellular P450BM3. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BENZENE
*PHENOL
*ESCHERICHIA coli
*CYTOCHROMES
*PHENYLALANINE
Subjects
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 57
- Issue :
- 38
- Database :
- Academic Search Index
- Journal :
- Angewandte Chemie International Edition
- Publication Type :
- Academic Journal
- Accession number :
- 131719338
- Full Text :
- https://doi.org/10.1002/anie.201804924