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Exosomes from Adipose-Derived Stem Cells Promotes VEGF-C-Dependent Lymphangiogenesis by Regulating miRNA-132/TGF-β Pathway.

Authors :
Wang, Xiaolei
Wang, Haichao
Cao, Jingli
Ye, Chen
Source :
Cellular Physiology & Biochemistry (Karger AG). Sep2018, Vol. 49 Issue 1, p160-171. 12p.
Publication Year :
2018

Abstract

<bold>Background/Aims:</bold> Lymphangiogenesis plays an important role in the pathogenesis of inflammatory bowel diseases (IBD), and vascular endothelial growth factor-C (VEGF-C) is a powerful lymphangiogenic factor. Adipose-derived stem cells (ADSCs) are a promising therapeutic modality for several diseases because ADSCs secret growth factors and exosomes, which modulate hostile microenvironments affected by diseases. However, the effect of exosomes on VEGF-C-dependent lymphangiogenesis and its mechanism remain unclear. <bold>Methods:</bold> ADSCs were cultured in media with or without recombinant VEGF-C and exosomes were extracted from conditioned medium (CM). Lymphatic endothelial cells (LECs) were treated with ADSCs-derived exosomes, then proliferation, migration and tube formation of LECs were assayed using cell counting Kit-8 (CCK-8), transwell chamber inserts and matrigel-based tube formation assay respectively. <bold>Results:</bold> We identified significantly higher levels of miR-132 in exosomes isolated from VEGF-C-treated ADSCs (ADSCs/VEGF-C) than in those from ADSCs control. miR-132 was directly transferred from ADSCs to the LECs by the mediation of exosomes. The exosomes from ADSCs/VEGF-C promoted LECs proliferation, migration, and tube formation more potently than the exosomes from ADSCs, whereas pretreatment of ADSCs with miR-132 inhibitor attenuates VEGF-C-dependent lymphangiogenic response. Finally we reveal that miR-132 promotes lymphangiogenic response by directly targeting Smad-7 and regulating TGF-β/Smad signaling. <bold>Conclusion:</bold> These data provide new insights into the role of ADSCs-derived exosomes as an important player in VEGF-C-dependent lymphangiogenesis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10158987
Volume :
49
Issue :
1
Database :
Academic Search Index
Journal :
Cellular Physiology & Biochemistry (Karger AG)
Publication Type :
Academic Journal
Accession number :
131635384
Full Text :
https://doi.org/10.1159/000492851