Nuciferine inhibits LPS-induced inflammatory response in BV2 cells by activating PPAR-γ.

Abstract Nuciferine, a bioactive component extracted from the lotus leaf , has been reported to have various anti-inflammatory effects. In the present study, we aimed to investigate the anti-inflammatory effects and mechanism of nuciferine on lipopolysaccharide (LPS)-stimulated BV2 microglia cells. The anti-inflammatory activity of nuciferine was measured by ELISA to detect the inflammatory mrdiators secretion in LPS-simulated BV2 microglia cells. The results demonstrated that nuciferine significantly inhibited LPS-induced TNF-α, IL-1β, PGE 2 and NO secretion. LPS-induced NF-κB activation was also suppressed by nuciferine. Further studies showed that nuciferine increased the expression of PPAR-γ. Functional aspects were analyzed using PPAR-γ specific inhibitor GW9662, which attenuated the LPS-induced secretion of proinflammatory mediators, such as TNF-α, IL-1β, PGE 2 , and NO. In conclusion, these results suggested that nuciferine activated PPAR-γ, which subsequently inhibited LPS-induced inflammation in BV2 cells. Highlights • Nuciferine significantly inhibited LPS-induced TNF-α, IL-1β, PGE 2 and NO production. LPS-induced NF-κB activation was suppressed by nuciferine. • Nuciferine increased the expression of PPAR-γ. • PPAR-γ specific inhibitor GW9662 attenuated the LPS-induced TNF-α, IL-1β, PGE 2 , and NO production. [ABSTRACT FROM AUTHOR]