川蔓藻内生及根际细菌多样性与抑菌活性研究.

The purpose of this study was to investigate the diversity of endophytic and rhizospheric bacteria of Ruppia maritima collected from Beihai City and antifungal activities against Penicilliosis marneffei. The endophytic and rhizosphere bacteria isolated from Ruppia maritima were analyzed by method of dilution butteron on plate. 16S rRNA gene sequencing was employed to explore species diversities and the effects of the crude extract from bacteria against Penicilliosis marneffei with agar diffusion method and spectrophotometer method. Total of 26 strains of endophytic bacteria and 31 strains of rhizosphere bacteria were isolated from Ruppia maritina. Thirteen species of endophytic bacteria were obtained and classified into twelve genera and ten families. Nineteen species of bacteria were isolated from rhizosphere and belonging to fourteen generas and nine families. And five strains in the rhizosphere were potential new genera. Crude extracts of eight strains showed inhibition effect against Penicilliosis marneffei, three of which had strong antifungal activities, and their MIC50 were(1.800 ± 0.045),(1.881 ± 0.061)and(1.604 ± 0.021)mg·mL-1. Endophytic and rhizospheric bacteria of Ruppia maritima are genetically diverse and most of them showed strong inhibition effects against Penicilliosis marneffei. [ABSTRACT FROM AUTHOR]