Interfering effects of bisphenol A on in vitro growth of preantral follicles and maturation of oocyes.

Aims In order to investigate the effects and mechanism of Bisphenol A (BPA) on the growth of preantral follicles and the maturation of oocytes in vitro , preantral follicles were harvested from mouse ovaries and in vitro cultured for 11 days with different concentrations of BPA (0, 4.5 and 45 μM) for calculating the percentages of antral follicles, denuded oocytes, degenerative oocytes and the maturation rate of oocytes, besides measuring the diameter of follicles and the thickness of cumulus cell layers. Methods The contents of estradiol (E 2 ) in the culture media on Day 4, 8 and 10 were detected by ELISA. The estrogen receptor (ER) expression, spindle morphology and chromosome distribution in oocytes on Day 10 and 11 were observed by immunofluorescence. Western blotting was used to detect the expressions of growth differentiation factor 9 (GDF-9), bone morphogenetic protein-15 (BMP-15), phosphorylated extracellular signal-regulated kinase 1 (p-Erk1) and phosphorylated Ca 2 + /calmodulin-dependent protein kinase II (p-CaMKII) in the oocytes. Results Compared with control, BPA (45 μM) significantly reduced percentages of antral follicles (9.25% vs. 91.17%, P   <   0.05 ) and the maturation rate of oocytes (7.61% vs. 79.83%, P   <   0.05 ), but increased the percentages of denuded oocytes (30.29% vs. 3.36%, P   <   0.05 ) and degenerative oocytes (45.70% vs. 2.45%, P   <   0.05 ). The diameter of follicles and the thickness of the cumulus cell layers were decreased significantly ( P   <   0.05 ). Moreover, BPA (45 μM) significantly decreased E 2 contents in the culture medium on Day 8 and 10 ( P <   0.05 ) and the expressions of ER, GDF-9 and BMP-15 in oocytes ( P   <   0.05 ). Furthermore, BPA (4.5 and 45 μM) treatment resulted in the abnormal spindle morphology and chromosome distribution, and the decreased expressions of p-Erk1 and p-CaMKII in the MII oocytes. Conclusion Together, these results clearly demonstrated BPA retarded the preantral follicle growth in vitro through interfering with the synthesis and secretion of E 2 and reducing the expressions of ER, GDF-9 and BMP-15, and led to the abnormal meioses of oocytes through reducing p-Erk1 and p-CaMKII expressions in the preantral follicles, which will help us to further unsderstand the mechanism of BPA exposure retarding in vitro growth of preantral follicles and maturation of oocyes. [ABSTRACT FROM AUTHOR]