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SBP1 is an adhesion-associated factor without the involvement of virulence in Streptococcus suis serotype 2.
- Source :
-
Microbial Pathogenesis . Sep2018, Vol. 122, p90-97. 8p. - Publication Year :
- 2018
-
Abstract
- Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that infects swine and humans with high mortality and morbidity. Although a number of virulence-associated factors have been reported, the understanding of the molecular mechanism underlying SS2 pathogenicity remains limited. Our previous studies revealed that srt BCD-associated protein 2’ (SBP2′) contributed to the pathogenesis of SS2, but the function of another member in the srt BCD cluster, srt BCD-associated protein 1 (SBP1) was still unknown. Here, we found that sbp 1 was widely distributed among high virulent SS2 strains, suggesting that sbp 1 may be involved in the pathogenesis of SS2. To investigate the function of SBP1, we firstly conducted Western blotting analyses to confirm that SBP1 was expressed in the high virulent SS2 strain ZY05719 both in vivo and in vitro , then constructed the deletion mutant of sbp 1 by homologous recombination. Bacterial adhesion assay, indirect immunofluorescence assay and protein binding assay all demonstrated that SBP1 was associated with adhesion of SS2 to HEp-2 cells. However, SBP1 did not influence the invasion, phagocytosis or intracellular survival of SS2. Furthermore, infection assays in vivo showed that inactivation of sbp 1 failed to impair the ability of SS2 to cause zebrafish and mouse mortality. Overall, these results indicate that SBP1 is an adhesion-associated factor without the involvement of virulence in Streptococcus suis serotype 2. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08824010
- Volume :
- 122
- Database :
- Academic Search Index
- Journal :
- Microbial Pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 131334470
- Full Text :
- https://doi.org/10.1016/j.micpath.2018.06.008