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Graphene-Based Raman Spectroscopy for pH Sensing of X-rays Exposed and Unexposed Culture Media and Cells.

Graphene-Based Raman Spectroscopy for pH Sensing of X-rays Exposed and Unexposed Culture Media and Cells.

Authors :
Camerlingo, Carlo
Verde, Alessandro
Manti, Lorenzo
Meschini, Roberta
Delfino, Ines
Lepore, Maria
Source :
Sensors (14248220). 7/1/2018, Vol. 18 Issue 7, p2242. 1p.
Publication Year :
2018

Abstract

Graphene provides a unique way of sensing the local pH level of substances on the micrometric scale, with important implications for the monitoring of cellular metabolic activities where proton excretion could occur. Accordingly, an innovative biosensing approach for the quantification of the pH value of biological fluids, to be used also with small amounts of fluids, was realized and tested. It is based on the use of micro-Raman spectroscopy to detect the modifications of the graphene doping level induced by the contact of the graphene with the selected fluids. The approach was preliminarily tested on aqueous solutions of known pH values. It was then used to quantify the pH values of cell culture media directly exposed to different doses of X-ray radiation and to media exposed to X-ray-irradiated cells. The Raman response of cells placed on graphene layers was also examined. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14248220
Volume :
18
Issue :
7
Database :
Academic Search Index
Journal :
Sensors (14248220)
Publication Type :
Academic Journal
Accession number :
131047578
Full Text :
https://doi.org/10.3390/s18072242