Back to Search Start Over

The Hippo pathway effector proteins YAP and TAZ have both distinct and overlapping functions in the cell.

Authors :
Plouffe, Steven W.
Lin, Kimberly C.
Moore 3rd, Jerrell L.
Tan, Frederick E.
Shenghong Ma
Zhen Ye
Yunjiang Qiu
Bing Ren
Kun-Liang Guan
Source :
Journal of Biological Chemistry. 7/13/2018, Vol. 293 Issue 28, p11230-11240. 11p.
Publication Year :
2018

Abstract

The Hippo pathway plays an important role in regulating tissue homeostasis, and its effectors, the transcriptional co-activators Yes-associated protein (YAP) and WW domain-containing transcription regulator 1 (WWTR1 or TAZ), are responsible for mediating the vast majority of its physiological functions. Although YAP and TAZ are thought to be largely redundant and similarly regulated by Hippo signaling, they have developmental, structural, and physiological differences that suggest they may differ in their regulation and downstream functions. To better understand the functions of YAP and TAZ in the Hippo pathway, using CRISPR/Cas9, we generated YAP KO, TAZ KO, and YAP/TAZ KO cell lines in HEK293A cells. We evaluated them in response to many environmental conditions and stimuli and used RNA-Seq to compare their transcriptional profiles. We found that YAP inactivation has a greater effect on cellular physiology (namely, cell spreading, volume, granularity, glucose uptake, proliferation, and migration) than TAZ inactivation. However, functional redundancy between YAP and TAZ was also observed. In summary, our findings confirm that the Hippo pathway effectors YAP and TAZ are master regulators for multiple cellular processes but also reveal that YAP has a stronger influence than TAZ. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219258
Volume :
293
Issue :
28
Database :
Academic Search Index
Journal :
Journal of Biological Chemistry
Publication Type :
Academic Journal
Accession number :
130763468
Full Text :
https://doi.org/10.1074/jbc.RA118.002715