卵巢癌组织中UHRF1蛋白的表达及其与卵巢癌细胞增殖和侵袭的关系.

Objective: To study the expression of ubiquitin-like with PHD and ring finger domains 1(UHRF1) protein in ovarian cancer tissue and the effect of UHRF1 on the proliferation and invasion of ovarian cancer cells. Methods: Ovarian cancer tissue and adjacent normal tissue were collected, the expresion of UHRF1 protein were detected by Westerrn blot. Ovarian cancer SKOV-3 cell was cultured in vitro, UHRF1 si RNA and negative control si RNA were transfected. Then the cell viability was assayed by CCK-8 kit, cell invasion capability was assayed by Transwell. Expressions of Cyclin D1, CDK6, MMP2 and MMP9 m RNA were assayed by FQ-PCR.Re sults: The expression of UHRF1 protein in ovarian cancer tissue was higher than that in adjacent normal tissue. The cell viability of SKOV-3 cells and number of migrated cells transfected by UHRF1 si RNA was significantly lower than that of SKOV-3 cell transfected by negative control si RNA, the expressions of Cyclin D1, CDK6, MMP2 and MMP9 m RNA were significantly decreased. Conclusions:The expression of UHRF1 protein was significantly increased in ovarian cancer; UHRF1 could promote the proliferation and invasion of ovarian cancer cell. [ABSTRACT FROM AUTHOR]