丹参酮II-A对万古霉素所致肾小管上皮细胞KIM-1 及TGF-β1 表达的影响.

Objective: To investigate the effects of tanshinon on the expression of KIM-1, TGF-β1 in vancomycin-induced HK-2 cells. Methods: HK-2 cells were randomly divided into six groups: normal saline(NS) group, model group, positive control group, high dose group, middle dose group and low dose group of TSII-A, The model were duplicated with addition of VAN(100 μg·L-1). The model group, positive control group, high dose group, middle dose group and low dose group of TSII-A were treated by saline solutions, Amifostine, panax notoginsenosides(100, 50, 25 mg·L-1). The cell viability was detected with MTT method, the content of MDA, NO and the activity of SOD, GSH-PX were measured and cell structure was observed. The concentration of KIM-1, TGF-β1 were measured by ELISA, the expressions of KIM-1, TGF-β1 m RNA were measured by RT-PCR. Results: Compared with the model group, the cell viability and activity of SOD, GSH-PX of TSII-A groups and positive control group were significantly increased(P0.05); the content of MDA,NO, KIM-1 were significantly decreased(P0.05); the expression of KIM-1 m RNA were significantly decreased(P0.05); the cell structure was significantly improved, there was no statistical difference(P0.05) in the concentration of TGF-β1 and the expression of TGF-β1 m RNA. Conclusions: TSII-A can promote the proliferation of HK-2 cells induced by VAN through relieving the oxidation stress. [ABSTRACT FROM AUTHOR]