The Reducing Power and the CO2 Fixation Activity of Isolated Chloroplasts.

Antimycin A, which stimulates the CO2 fixation activity of isolated intact spinach (Spinacia oleracea L.) chloroplasts, was shown to stimulate also the oxygen evolution in the presence of oxaloacetate, as do the uncouplers. Some uncouplers (tetramethylethylenediamine, NH4Cl) were shown to stimulate the CO2 fixation activity, and to decrease the intrachloroplastic ATP levels, like antimycin A. the intrachloroplastic NADPH levels were not affected by the inhibitors studied.