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The ESCRT CHMP2B acts as a diffusion barrier on reconstituted membrane necks.
- Source :
-
Journal of Cell Science . 7/1/2018, Vol. 131 Issue 13, p1-49. 49p. - Publication Year :
- 2018
-
Abstract
- ESCRT-III family proteins catalyze membrane remodeling processes that stabilize and constrict membrane structures. It was proposed that stable ESCRT-III complexes containing CHMP2B could establish diffusion barriers at post-synaptic spine neck. In order to better understand this process, we developed a novel method based on fusion of Giant Unilamellar Vesicles to reconstitute ESCRT-III proteins inside GUVs, from which membrane nanotubes are pulled. The new assay ensures that ESCRT-III proteins polymerize only when they become exposed to physiologically relevant membrane topology mimicking the complex geometry of post-synaptic spines. We establish that CHMP2B, both as full-length and with a C-terminal deletion (ΔC), preferentially binds to membranes containing PI(4,5)P2. Moreover, we show that CHMP2B preferentially accumulates at the neck of membrane nanotubes, and provide evidence that CHMP2B-ΔC prevents the diffusion of PI(4,5)P2 lipids and membranebound proteins across the tube neck. This indicates that CHMP2B polymers formed at a membrane neck may function as a diffusion barrier, a potential important function of CHMP2B to maintain synaptic spine structures. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEINS
*BIOLOGICAL membranes
*TOPOLOGY
*SPINE
*NANOTUBES
*LIPIDS
Subjects
Details
- Language :
- English
- ISSN :
- 00219533
- Volume :
- 131
- Issue :
- 13
- Database :
- Academic Search Index
- Journal :
- Journal of Cell Science
- Publication Type :
- Academic Journal
- Accession number :
- 130605277