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Analysis of Escherichia coli STs and resistance mechanisms in sewage from Islamabad, Pakistan indicates a difference in E. coli carriage types between South Asia and Europe.
- Source :
-
Journal of Antimicrobial Chemotherapy (JAC) . Jul2018, Vol. 73 Issue 7, p1881-1785. 5p. - Publication Year :
- 2018
-
Abstract
- <bold>Objectives: </bold>To discover the Escherichia coli STs and associated resistance mechanisms in the community in Islamabad, Pakistan by analysis of E. coli isolates in sewage.<bold>Methods: </bold>One hundred and ten E. coli were isolated from sewage across the city of Islamabad without antibiotic bias and confirmed as E. coli by MALDI-TOF MS. Isolates were characterized by fumC/fimH (CH) typing and core-genome MLST. Resistance mechanisms, virulence genes, phylotypes and plasmid incompatibility types were determined in a subset of isolates by in silico analysis. The genomic position of blaCTX-M-15 was determined using S1-PFGE, probing and Nanopore MinION sequencing.<bold>Results and conclusions: </bold>The most prevalent STs were ST394, ST10 and ST648, accounting for 39% of all isolates collected and were found at many sites across Islamabad. Carbapenemase genes were absent and only a single isolate of ST131 was found. The most prevalent resistance mechanisms were qnrS1 and blaCTX-M-15, with blaCTX-M-15 penetrating many STs and found in 31% of all collected isolates. However, the majority of the successful STs were blaCTX-M-15 negative indicating that resistance is not the main driver of prevalence. Twenty-three percent of blaCTX-M-15 genes were chromosomally encoded and large ISEcp1-mediated insertions included qnrS1 and several plasmid genes. In all chromosomally encoded isolates no plasmid copies of blaCTX-M-15 were found. The most prevalent ST (ST394) contained many enteroaggregative E. coli virulence genes and the fimH30 variant allele previously linked to the success of ST131. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICROBIAL virulence
*URINARY tract infections
*DRUG resistance in microorganisms
*PATHOGENIC microorganisms
*BETA lactamases
*BACTERIAL proteins
*BACTERIOPHAGE typing
*COMPARATIVE studies
*ESCHERICHIA coli
*GENES
*GENOMES
*HYDROLASES
*RESEARCH methodology
*MEDICAL cooperation
*MICROBIAL sensitivity tests
*PULSED-field gel electrophoresis
*RESEARCH
*SEWAGE
*TOXINS
*EVALUATION research
*SEQUENCE analysis
Subjects
Details
- Language :
- English
- ISSN :
- 03057453
- Volume :
- 73
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Journal of Antimicrobial Chemotherapy (JAC)
- Publication Type :
- Academic Journal
- Accession number :
- 130252291
- Full Text :
- https://doi.org/10.1093/jac/dky109