Wnt/β-catenin、ＲANKL/LGＲ4 通路因子在骨质疏松性骨折端的表达.

Objective To detect the expression of key factors of Wnt /β-catenin and ＲANKL /LGＲ4 pathways in fracture tissues of patients with osteoporotic fracture ( OPF) ． Methods Fifty-six patients with femoral fractures from January 2016 to July 2017 in our hospital were selected． Twenty-seven cases were divided into OPF group and 29 cases were divided into control group． Surgery was performed in 3 － 7 days． Bone tissue ( more than 200 mg) from the fracture area was collected and stored in liquid nitrogen． Total ＲNA was extracted from bone tissue using liquid nitrogen grinding ＲNAiso Plus method ． The difference of mＲNA expression of each factor was compared between the two groups using ＲT-PCＲ technique． Total protein was extracted from bone tissue using liquid nitrogen grinding and pyrolysis liquid． The protein expression difference of each factor was detected and compared between the two groups using Western blotting technique． Ｒesults ＲT-PCＲ and Western blotting result showed that LＲP5，β-catenin，Ｒunx2， C-myc，and LGＲ4 decreased，but ＲANKL increased in OPF group，comparing to those in the control group ( P ＜ 0. 05 ) ． Conclusion OPF is associated with the inhibition or weakening of osteogenic factors LＲP5，β-catenin，Ｒunx2，and C-myc in Wnt /β-catenin osteogenic signaling pathway，the increase of osteoclastic factor ＲANKL in ＲANKL /LGＲ4 osteoclastic signaling pathway，and the inhibition of osteoclastic factor LGＲ4． The occurrence and treatment of OPF can be intervened by regulating the expression of various factors． [ABSTRACT FROM AUTHOR]