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Epistatic interactions of deletion mutants in the genes encoding the F1-ATPase in yeast Saccharomyces cerevisiae.
- Source :
-
EMBO Journal . 1/1/99, Vol. 18 Issue 1, p58-64. 7p. - Publication Year :
- 1999
-
Abstract
- The F1-ATPase is a multimeric enzyme (α3β3γδε) primarily responsible for the synthesis of ATP under aerobic conditions. The entire coding region of each of the genes was deleted separately in yeast, providing five null mutant strains. Strains with a deletion in the genes encoding α-, β-, γ- or δ-subunits were unable to grow, while the strain with a null mutation in ε was able to grow slowly on medium containing glycerol as the carbon source. In addition, strains with a null mutation in γ or δ became 100% ρ0/ρ­ and the strain with the null mutation in γ grew much more slowly on medium containing glucose. These additional phenotypes were not observed in strains with the double mutations: ΔαΔγ, ΔβΔγ, Δatp11Δγ, ΔαΔδ, ΔβΔγ or Δatp11Δδ. These results indicate that ε is not an essential component of the ATP synthase and that mutations in the genes encoding the α- and β-subunits and in ATP11 are epistatic to null mutations in the genes encoding the γ- and δ-subunits. These data suggest that the propensity to form ρ0/ρ­ mutations in the γ and δ null deletion mutant stains and the slow growing phenotypes of the null γ mutant strain are due to the assembly of F1 deficient in the corresponding subunit. These results have profound implications for the physiology of normal cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02614189
- Volume :
- 18
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- EMBO Journal
- Publication Type :
- Academic Journal
- Accession number :
- 13003796
- Full Text :
- https://doi.org/10.1093/emboj/18.1.58