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Valency‐Controlled Framework Nucleic Acid Signal Amplifiers.

Authors :
Liu, Qi
Ge, Zhilei
Mao, Xiuhai
Zhou, Guobao
Zuo, Xiaolei
Shen, Juwen
Shi, Jiye
Li, Jiang
Wang, Lihua
Chen, Xiaoqing
Fan, Chunhai
Source :
Angewandte Chemie. 6/11/2018, Vol. 130 Issue 24, p7249-7253. 5p.
Publication Year :
2018

Abstract

Abstract: Weak ligand–receptor recognition events are often amplified by recruiting multiple regulatory biomolecules to the action site in biological systems. However, signal amplification in in vitro biomimetic systems generally lack the spatiotemporal regulation in vivo. Herein we report a framework nucleic acid (FNA)‐programmed strategy to develop valence‐controlled signal amplifiers with high modularity for ultrasensitive biosensing. We demonstrated that the FNA‐programmed signal amplifiers could recruit nucleic acids, proteins, and inorganic nanoparticles in a stoichiometric manner. The valence‐controlled signal amplifier enhanced the quantification ability of electrochemical biosensors, and enabled ultrasensitive detection of tumor‐relevant circulating free DNA (cfDNA) with sensitivity enhancement of 3–5 orders of magnitude and improved dynamic range. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00448249
Volume :
130
Issue :
24
Database :
Academic Search Index
Journal :
Angewandte Chemie
Publication Type :
Academic Journal
Accession number :
130001388
Full Text :
https://doi.org/10.1002/ange.201802701