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Synthesis and characterization of tri(ethylene oxide)-attached poly(amidoamine) dendrimer layers on gold
- Source :
-
Journal of Colloid & Interface Science . Jun2004, Vol. 274 Issue 1, p41. 8p. - Publication Year :
- 2004
-
Abstract
- This paper describes the synthesis of a tri(ethylene oxide)-attached fourth-generation poly(amidoamine) dendrimer (EO3-dendrimer) and the characterization of its layers on gold. NMR analysis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry revealed that about 61 amine groups of a G4 PAMAM dendrimer were covalently conjugated with tri(ethylene oxide) units, accounting for a 95% modification level. Layers of the EO3-dendrimer were formed on gold, and the resulting surface was characterized by infrared reflection absorption spectroscopy, ellipsometry, and contact angle goniometry. The EO3-dendrimer resulted in more hydrophilic and less compact layers with no substantial deformation of the molecule during layer formation by virtue of the EO3 units, compared to a PAMAM dendrimer. Interestingly, the specific binding of avidin to the biotinylated layers of the EO3-dendrimer approached a surface density of <f>5.2±0.2</f> ng mm−2, showing about 92% of full surface coverage. The layers of the EO3-dendrimer were found to be more resistant to nonspecific adsorption of proteins than PAMAM dendrimer layers when bovine serum albumin and serum proteins were tested. [Copyright &y& Elsevier]
- Subjects :
- *ETHYLENE synthesis
*INSECTICIDES
*DISINFECTION & disinfectants
*EPOXY compounds
Subjects
Details
- Language :
- English
- ISSN :
- 00219797
- Volume :
- 274
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Colloid & Interface Science
- Publication Type :
- Academic Journal
- Accession number :
- 12984109
- Full Text :
- https://doi.org/10.1016/j.jcis.2003.11.061