Back to Search
Start Over
Immunohistopathologic evaluation of choroidal neovascular membranes following Verteporfin-photodynamic therapy
Immunohistopathologic evaluation of choroidal neovascular membranes following Verteporfin-photodynamic therapy
- Source :
-
American Journal of Ophthalmology . May2004, Vol. 137 Issue 5, p914-923. 10p. - Publication Year :
- 2004
-
Abstract
- : PurposeTo evaluate the vascularization and proliferative activity in choroidal neovascular membranes due to age-related macular degeneration after verteporfin photodynamic therapy and submacular removal.: DesignInterventional case series.: MethodsIn a retrospective review of seven patients who underwent removal of subfoveal classic choroidal neovascular membranes after treatment with photodynamic therapy 3 to 146 days earlier, membranes were stained for CD 34, CD 105, and Ki-67 and correlated with clinical pictures and fluorescein angiography.: ResultsFluorescein angiography performed on the day of surgery disclosed nonperfusion of the treated area 3 days after photodynamic therapy, but perfusion and leakage were seen at greater post–photodynamic therapy intervals. Membranes excised 3 days after photodynamic therapy showed CD34 and CD105 positive, mostly occluded vessels. The endothelial cells appeared damaged. Ki-67 activity was low. In membranes excised 34 to 146 days after photodynamic therapy, all vessels appeared patent and were lined by healthy endothelial cells with strong expression of CD34 and CD105. Ki-67 expression was elevated after 34 days but decreased thereafter.: ConclusionPhotodynamic therapy did not cause a general or complete occlusion of vessels within the choroidal neovascular membranes, as suggested by fluorescein angiography 3 days postintervention, but the endothelial cells appeared to be severely damaged. Proliferative activity within these specimens was reduced. At longer intervals after photodynamic therapy, the fibrovascular tissue seemed to recover; perfusion, hyperfluorescence, and leakage of the choroidal neovascular membranes could be detected by fluorescein angiography. The clinical appearance showed a correlation with the immunohistologic characteristics of an increased proliferative activity and patent vascularization. [Copyright &y& Elsevier]
- Subjects :
- *PHOTOCHEMOTHERAPY
*FLUORESCENCE angiography
*RETINAL degeneration
*DISEASES in older people
*FAMILIES & psychology
*PALLIATIVE treatment
*PSYCHOLOGY of caregivers
*COMPARATIVE studies
*ECONOMIC aspects of diseases
*RESEARCH methodology
*MEDICAL quality control
*MEDICAL care costs
*MEDICAL cooperation
*MEMORY
*RESEARCH
*SOCIAL support
*EVALUATION research
*ECONOMICS
Subjects
Details
- Language :
- English
- ISSN :
- 00029394
- Volume :
- 137
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- American Journal of Ophthalmology
- Publication Type :
- Academic Journal
- Accession number :
- 12983774
- Full Text :
- https://doi.org/10.1016/j.ajo.2003.12.049